E-ISSN 2229-4619
  International Journal of Pharmaceutical Studies and Research
                                                                      -An Open Access Peer Reviewed e-Journal

 

 
 

DEVELOPMENT AND VALIDATION OF A SIMPLE HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR DETERMINATION OF KETOPROFEN IN HUMAN PLASMA

 

Authors: Jiyauddin Khan, Kah Hay Yuen, Ng Bee Hon, Wong Jia Woei, Samer AL-Dhalli, Gamal Osman Elhassan, Mallikarjun Chitneni, Kaleemullah Mohammed1, Junainah, Eddy Yusuf  and Fadli

  IJPSR/Volume II/ Issue IV/ October-December, 2011/1-5

ABSTRACT
The present study was aimed to develop and validate a simple reversed-phase high performance liquid chromatographic method with UV detection at 260 nm for the determination of ketoprofen in human plasma. The sample preparation involved a simple protein precipitation method using perchloric acid along with acetonitrile as the deprotenizing agents. Chromatographic separation was achieved isocratically by using C-18 column (5 Ám, 250 x 4.6 mm ID). The mobile phase was comprised of 0.05M disodium hydrogen phosphate buffer and acetonitrile (70:30, v/v) adjusted to pH 6.5 with glacial acetic acid. Analysis was run at a flow rate of 1.0 mL/min with 50 ÁL injection volume and the samples were quantified using peak height measurement. The calibration curve was linear in the range of 156.25-20000 ng/mL with correlation coefficient (r) of 0.9999 (▒ 0.0001). The within-day accuracy ranged between 83.80 and 112.56% with precision between 3.41 and 7.07%. The between-day accuracy ranged between 91.57 and 99.84% with precision between 1.62 and 16.06%. The recovery ranged between 96.45 and 102.92% with precision between 2.90 and 9.92%, respectively. The values of accuracy, precision and recovery obtained were within the acceptable limits as proposed by USFDA Bioanalytical Guidelines. In conclusion, the developed and validated method can be used to estimate plasma ketoprofen concentration after oral administration of Pharmaceutical dosage forms.

PRELIMINARY PHYTOCHEMICAL STUDIES ON LEAVES OF PHYLLANTHUS SPECIES (EUPHORBIACEAE), USED BY THE LOCAL TRIBALS OF ANDHRA PRADESH

  Authors: C. Lakshmi Narasimhudu and R.R.Venkata Raju*
 

IJPSR/Volume II/ Issue IV/ October-December, 2011/6-9

ABSTRACT
The present paper deals with preliminary phytochemical screening of Phyllanthus species (Euphorbiaceae), used by the local tribals for different human ailments. The personal interviews conducted with local tribal practitioners and critical review of literature yielded 10 species of Phyllanthus as an effective remedy for jaundice, dysentery, abortifacient, allergy, boils, diarrhea, dropsy, eruptions, genito-urinary diseases, etc. The preliminary phytochemical screening was conducted on the leaf samples, which was used as drug for different ailments, and composition of various groups of constituents was discussed.

RECENT ADVANCEMENT IN CAUSAL INFERENCE

  Authors: ArunKumar N and Venkatesan P
  IJPSR/Volume II/ Issue IV/ October-December, 2011/10-19

ABSTRACT
This paper attempts to summarise latest development in causal inference and highlight the transition of conventional statistical analysis to causal analysis. Review the assumptions that forms the basis of all causal inferences, the jargons used in devising these assumptions, the conditional character of all causal and counterfactual claims, and the techniques that have been developed for the evaluation of such claim. Pearl (2000a) illustrated using a general theory of causation based on the Structural Causal Model (SCM), by unifying other approaches to causation, and provided a coherent mathematical foundation for the analysis of causes and counterfactuals. We review the formal and conceptual relationships between the structural and potential-outcome frameworks.

CAMEL AND DONKEY MILK BASED NUTRITIVE POWDER: A CHEAPER ALTERNATIVE OF HUMAN MILK
  Authors: Madhu Rathore , Mukesh Sharma,  Manorama Mahich
  IJPSR/Volume II/ Issue IV/ October-December, 2011/20-22

ABSTRACT:
Existing milk based product causes various health implications on infants and new born. Hence in the present work, camel milk and ass milk was studied for their nutritive value. Both are already known for their various medicinal properties.  Study suggested that both milk have good protein and easily digestible sugar. In addition both were observed to be good in mineral content. Hence milk powder composed of camel and ass milk could be best nutritive alternative of human milk and other baby foods and could be employed after clinical trials.

NANOSUSPENSION DRUG DELIVERY

  Authors: Jain Amit K., Dayal Rakesh
  IJPSR/Volume II/ Issue IV/ October-December, 2011/23-27

ABSTRACT:
Nanosuspension technology offers novel solution for these poorly soluble drugs. Nanosuspensions are sub-micron colloidal dispersion of pure particles of drug, which are stabilised by surfactants. Nanosuspensions differ from nanoparticles, which are polymeric colloidal carriers of the drug and solid lipid nanoparticles, which are lipidic carrier of drug. Nanosuspension technology can be used to improve the stability as well as the bioavailability of poorly soluble drugs. Nanosuspensions are biphasic systems consisting of pure drug particles dispersed in an aqueous vehicle, stabilized by surfactants. These are simple to prepare and are more advantageous than other approaches. Techniques such as wet milling, high-pressure homogenization, emulsification-solvent evaporation and super critical fluid have been used in the preparation of nanosuspensions. It has the advantage of delivery by various routes, including oral, parenteral, pulmonary and ocular routes. The present article reviews the current methods used to prepare nanosuspensions and their application in drug delivery.